SEPARATION OF STEREOISOMERS OF DIFENOCONAZOLE IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY USING THE MASS SPECTROMETRY COMPATIBLE MOBILE PHASES

Author: Natia Tchanturia
Co-authors: Academician Bezhan Chankvetadze
Keywords: HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, DIFENOCONAZOLE
Annotation:

Difenoconazole is a fungicide used in agriculture to control various fungal diseases, for example in fruits, vegetables, cereals and other field crops. Difenoconazole acts by inhibition of demethylation during ergosterol synthesis [1].Difenoconazole has two chiral carbon centers and thus 4 stereoisomers. The goal of the present study was to study a separation of stereoisomers of difenoconazole in high-performance liquid chromatography by using polysaccharide-based chiral columns. In this study, we investigated the separation of stereoisomers of difenoconazole with mass spectrometer-compatible mobile phases. Based on the obtained results, we made some conclusions about the influence of the mobile phase on the retention and separation of difenoconazole stereoisomers on chiral columns of polysaccharide nature.