Examining the functional activity of cytochrome C under prolonged glycation conditions.

Author: Davit Davit27
Keywords: long-term glycation, cytochrome C, cyclic voltammetry, electron transport
Annotation:

The objective of this study is to assess the impact of long-term glycation on the cellular component's influence on the functional activity of cytochrome C at the electrode under modeled experimental conditions. Cytochrome C holds significance not only for its crucial biological role in electron transport (in ATP synthesis) but also for its signaling function in cell type II internal apoptosis. In simpler terms, cytochrome C is involved in both vital and programmed cell death processes. In this research, the modern cyclic voltammetry method was employed to observe both the oxidation and reduction processes of cytochrome C in artificial conditions (on a conductive electrode) against the background of long-term glycation. This approach aims to deepen the understanding of the physical and chemical characteristics of the test object. It is noteworthy that in previous research conducted in the 2010s, authors utilized spectroscopy and concluded that the function of cytochrome C decreases as a result of long-term glycation. Within the scope of our work, the results of the aforementioned research were partially confirmed (for details, refer to the master's thesis). Recent studies have indicated that long-term and advanced glycation end products play a crucial role in the development of diabetes and age-related diseases. Therefore, investigating how glycation affects the proper functioning of one of the key proteins is a highly relevant topic in modern biomedicine. This research may pave the way for new and promising studies in the future.